The severe emphysema noticed in Macho B’s left, hind leg was cultured at his necropsy. The following aerobic organisms were found: Escherichia coli (found in gut; produces vitamin K2 and prevents the establishment of pathogenic bacteria within the intestine), Enterococcus species (normal flora of intestinal tract), and Acinetobacter calcoaceticus-baumannii (bacteria found in soil, can lead to an infection in a body after severe trauma).
This subcutaneous emphysema was dissecting through muscle bellies of the hind limbs. It was also noted that Macho B had moderate muscle atrophy in both hind limbs. The cause for the severe emphysema was never determined. It appears it was NOT an infection as his white blood cell count was normal.
The taxidermist, Marc Plunkett, contracted by AZGFD to skin Macho B and preserve his hide noticed this serious hind leg wound that was “oozing” pus type substances. He noted the wound did not smell bad and seemed to be an internal wound rather than a puncture wound. He also noted a wound he thought was from a dart entry on the same leg. Mr. Plunkett spoke with Tony Davis of the AZ Daily Star about the wound and that article can be found in the blogroll. Below is a picture of the wound taken by Mr. Plunkett. Thanks to Wikipedia for having posts on medical terms which I used here.